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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and so might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two added splice variants were claimed but thorough nucleotide sequence information will not be but offered.
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Blocking peptides are peptides that bind precisely towards the goal antibody and block antibody binding. These peptide commonly consists of the epitope recognized from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to be certain the best possible outcomes are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the best possible final results are...
Antibodies sure to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Concentrate on Details Cyclin E belongs to the very conserved cyclin family, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit check here of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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